Direct, derivative spectrophotometric determination of micro amounts of Vanadium (V) by 5-bromo salicylaldehyde isonicotinoyl hydrazone

A simple and highly selective spectrophotometric method has been developed for the determination of trace amounts of V (V) using newly synthesized reagent 5-bromo salicylaldehyde isonicotinoyl hydrazone (5BrSAINH) in aqueous DMF medium. Vanadium (V) forms a brown colored soluble complex with 5-BrSAINH in the pH range 1.0-5.0. The complex shows maximum absorbance at 400nm in the pH range 4.5-5.5 where the reagent blank shows negligible absorbance. Hence analytical studies were carried out at 400nm and at pH 5.0. Beer’s law was obeyed in the range 0.51-5.1μgmL of V (V). The molar absorptivity and sandell’s sensitivity for the colored solution were found to be 1.25 x 10 L.molcm and4.0 X10μgcm respectively. The brown colored complex has 1:1 stoichiometry. The interference effect of various diverse ions has been studied .The stability constant of the complex was determined as 2.70 X 10 by Job’s method. A second order derivative spectrophotometric method was developed for the determination of vanadium (V) which was found to be more sensitive than zero order method. The developed methods have been employed for the determination of vanadium (V) in water, human hair and rice samples.